羧甲基菊粉的制备及其抗氧化活性研究
Study on preparation and antioxidant activity of carboxymethyl inulin
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摘要: 采用氢氧化钠-氯乙酸反应体系,以异丙醇作为溶剂制备羧甲基菊粉（CMI）,并研究其抗氧化活性。以取代度为指标,通过单因素及正交实验优化CMI制备条件,并对其·OH清除活性和红外光谱进行研究。结果表明,CMI制备的最佳条件为碱化时间3min,氯乙酸用量6.75g,异丙醇用量4mL,醚化时间15min,该条件下取代度为1.3327。抗氧化活性研究表明,CMI具有一定的·OH清除能力,且与浓度呈正相关效应,但与取代度之间却无确定关系。红外光谱分析表明CMI的制备工艺可行,应用前景广阔。Abstract: Carboxymethyl inulin (CMI) was prepared in the reaction system of NaOH-chloroacetic acid with isopropanol as the solvent.The antioxidative activity of CMI was studied.The preparation technology of CMI was optimized by single factor and orthogonal experiments with the evaluation index of substitution degree.The scavenging activities against hydroxyl free radicals and infrared spectrum of CMI were studied.The results showed that the suitable conditions were as follows:alkalization time 3min, chloroacetic acid amount 6.75g, isopropanol amount 4mL, etherification time 15min.The maximum substitution degree of CMI was 1.3327 under the optimum reaction condition.Meanwhile, CMI had strong scavenging capacities against hydroxyl free radicals with a dose-dependent effect.Structure confirmation by infrared spectroscopy (IR) proved that the preparation method of CMI was feasible.
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