两代繁殖实验研究牛初乳粉对大鼠生殖发育的影响
Effect of bovine colostrums powders on reproductive development in male and female rats assessed by a two-generation reproduction
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摘要: 为探讨围产期接受牛初乳粉对子代生殖发育的影响,设计了两代繁殖实验,分析亲代和子代雌雄鼠血清激素水平,生殖器官发育和生殖受孕变化。结果表明:食用含有牛初乳粉的饲料后,亲代和子代雌鼠生殖受孕指标及雄鼠精子密度和活率与空白组相比无显著差异(p>0.05)。亲代和子代雄鼠血清激素水平与空白组相比无显著差异(p>0.05),但亲代雌鼠血清中催乳素和孕酮显著低于空白组(p<0.05),子一代血清中催乳素显著低于空白组(p<0.05)。子二代雌鼠F2a生殖器间距与空白组相比显著降低(p<0.05),子二代F2b的阴道开口时间推迟(p<0.05),不能确定是偶然发生还是受生理影响所致,需要重复实验的验证。Abstract: To examine the effect of bovine colostrum powder on the reproductive development and reproductive ability of offspring rats when the exposure began in uteri, the two-generation reproduction study was designed.The hormone levels in serum, the development of reproductive organs and reproductive date were detected.There were no significant differences in fertility or reproductive organ development of both parents and offspring female pups, or in the germ cell numbers and the germ activity of both parents and offspring male pups between bovine colostrum powder group and control group (p>0.05) .There were no significant differences in the hormone levels of parents and offspring male pups between two groups (p>0.05) .However, the progesterone content of parents female rats and the prolactin contents of parents and offspring female pups in bovine colostrum powder group were significant lower than those of control group (p<0.05) .The anogenital distance of F2a female pups in bovine colostrum powder group was shorten (p<0.05) , and the vaginal open of F2b female pups in bovine colostrum powder group were delayed comparing with control group (p<0.05) .It was unknown whether they occurred by chance or was biologically significant until this treatment effect is replicated.