Abstract: Purpose:To optimize the extraction conditions for polysaccharides from truffles.Methods:Based on the single-factor experiments, the Box-Behnken center-united experimental design for 3 factors were used to determine the extraction condition.Results:Within the tested ranges, the impact of three process parameters on extraction yield of polysaccharides from big to small was extraction temperature, liquid-to-solid ratio and extraction time.The optimum extraction temperature was 90℃, extraction time was 60min and liquid-to-solid ratio was 33:1 for polysaccharides extraction.The predictive maximum yield of polysaccharides extraction rates was 13.45% and the polysaccharide content was 70.75%, the average yield of polysaccharides in three validation experiment under the optimum process conditions was 13.32% and the relative error was 0.96%, the actual content was 71.55% and the relative error was 1.13%.Conclusion:The response surface optimized condition for extraction polysaccharides improved extraction efficiency, suggested which is suitable for the extraction of polysaccharides from truffles because of its reliability and briefness.