Abstract: An ion chromatographic method had been developed for the determination of sucrose, glucose and lactose in toffee.The optimal chromatographic conditions were investigated for the separation on METROSEP CARB 1 (150mm×4.0mm) anion exchange column.Pulsed amperometric detector was used for detection.Column temperature was 32℃.The separation was performed using 37.0mmol/L NaOH at a flow rate of 1.0mL/min.The total analysis time was 20min.The linear ranges of sucrose, glucose and lactose were 1.0～60.0mg/L, respectively.Their correlation coefficients were 0.9996, 0.9998 and 0.9996.The detection limits were 0.091, 0.014 and 0.083mg/L, correspondingly.The relative standard deviation was between 1.42% and 5.17%.The recoveries were between 91.27% and 101.31%.The results of the samples showed that glucose content of white rabbit toffee was 1.56%, sucrose content was 21.40%, lactose content was 3.75%.Glucose content of Wangzai toffee was 1.17%, sucrose content was 30.29%, lactose content was 3.30%.Glucose content of golden monkey toffee was 1.08%, sucrose content was 20.86%, lactose content was 3.31%.In the application of the method to the analysis of toffee satisfactory results were obtained.At the same time, this method was characteristic of simple operation, the accurate results and shorter analysis time.It could provide a simple and efficient method for the determination of toffee.