Abstract: Objective:To study the sleep improvement effect of instant asparagus powder on mice.Methods:Healthy KM male mice with the body weight of 18²2g were divided into groups randomly, then mice were gavaged with the different dosage of instant asparagus powder and jujubosided respectively everyday, and the direct sleep effect of the test material on mice were observed.30d later, the hypnosis test under subthreshold dose of Sodium Pentobarbital, the incubation period test with Barbital Sodium and the sleep extending test with Sodium Pentobarbital were conducted.Results:The sleep rate of low, medium and high dose group of instant asparagus powder were 25.00%, 33.33% and 41.67% respectively, significantly raised the sleep rate comparing with the control group, it showed that the instant asparagus powder had hypnotic effect under the threshold hypnotic dosage of pentobarbital sodium.The sleeping incubation period of low, medium and high dose group of instant asparagus powder were 23.09, 20.29, 20.22min respectively, compared with the control group time 32.36min, instant asparagus powder significantly shorten the sleep incubation period of mice.The sleep time of low, medium and high dose group of instant asparagus powder were 35.89, 40.62, 45.78min respectively, the result showed that instant asparagus powder could significantly prolong sleep time that induced by pentobarbital sodium.Conclusion:Instant asparagus powder could improve the sleeping quality of mice.