沙棘籽粕蛋白的碱酶两步法提取工艺及功能性研究

Study on extraction of seabuckthorn seed meal protein by alkaline extraction and enzymatic hydrolysis and on functional properties

摘要: 以沙棘籽粕为原料,采用碱提酸沉法提取沙棘籽粕蛋白,得到最佳提取工艺参数为:pH10、料液比1:12、温度60℃、时间60min。后用碱性蛋白酶对碱提残渣中的蛋白进行水解,最适提取条件为:pH8.5、碱性蛋白酶加酶量240U/g、料液比1:10、60℃、60min。通过碱提、酶法两步提取后,总提取率为67.24%。并对其乳化活力和乳化稳定性等功能性质进行了研究,表明性质受pH、温度、盐离子浓度等环境因素影响大,在等电点pH5.0附近时乳化活力和乳化稳定性的数据最低。

Abstract: Firstly, alkaline extraction and acid precipitation process of seabuckthorn seed meal protein (SSMP) was studied.The optimal parameters were determined as follows:pH10, ratio of seabuckthorn seed meal (SSM) to water 1:12, 60℃, 60min.Secondly, the residue was further hydroyzed by alcalase.The optimum condition of enzymatic hydrolysis was alcalase addition 240U/g, ratio of residue to water 1:10, 60℃, 60min.And its emulsifying activity and foaming activity were studied.The results showed that its functional propertises were affected by the environmental factors, such as alkali extraction pH, extraction temperature and ionic strengths.Most of indexes of functional properties at pH5.0 were the lowest.