粗壮脉纹孢菌发酵稻谷加工副产物生产类胡萝卜素培养基的优化

Optimization of culture medium composed of rice by-products for carotenoid production by fermentation of Neurospora crassa

摘要: 采用响应面法对粗壮脉纹孢菌发酵稻谷加工副产物（砻糠和脱脂米糠）生产类胡萝卜素的培养基进行了优化。通过Plackett-Burman设计法,研究了脱脂米糠、（NH4）2SO4、KH2PO4、MgSO4、MnSO4、CaCl2等6个因素对粗壮脉纹孢菌产类胡萝卜素的影响,筛选显著影响因素。然后通过响应面法优化发酵培养基组成。研究表明,脱脂米糠和（NH4）2SO4为类胡萝卜素发酵的主要影响因素。得到优化培养基为:脱脂米糠64.58%、砻糠33.85%、（NH4）2SO41.57%。在优化发酵工艺下,色素产量达到366mg/kg培养物。

Abstract: The culture medium composed of rice by-products (rice chaff and defatted rice bran) for carotenoid production by Neurospora crassa was optimized through statistical experiment design and data analysis. The defatted rice bran, (NH4) 2SO4, KH2PO4, MgSO4, MnSO4 and CaCl2 were evaluated as factors affecting carotenoid biosynthesis by Neurospora crassa, and the significant factors were screened by a Plackett-Burman design. Then the culture medium was optimized by response surface methodology. Results indicated that defatted rice bran and (NH4) 2SO4 were the significant factors on carotenoid production. The optimal culture medium consisted of 64.58% defatted rice bran, 33.85% rice chaff and 1.57% (NH4) 2SO4 at which the yield of carotenoids was gotten up to 366mg/kg culture using the optimized fermentation parameters.