响应面法优化小球藻培养基
Optimization of medium for Chlorella vulgaris by response surface analysis
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摘要: 为了提高小球藻的生物量,对BG11培养基的成分进行了响应面优化。通过单因素实验筛选出了适合小球藻生长的最佳碳源、氮源分别为葡萄糖和尿素,并发现适量添加海绿素可显著促进小球藻的生长。利用Minitab软件设计Plackett-Burman实验筛选出了影响小球藻生长的三个最重要因子;通过Box-Behnken实验及响应面分析确定了三个因子的最佳浓度:磷酸氢二钾58mg/L,硫酸镁162mg/L,海绿素198μL/L。用优化后的培养基培养小球藻,48h后的藻细胞干重达10.09g/L,比优化前提高了61.2%,油脂及蛋白质产量分别达3.62和3.81g/L。Abstract: In order to improve the biomass yield of Chlorella vulgaris, optimization of BG11 medium was carried out by response surface analysis. The optimal carbon and nitrogen sources for C. vulgaris were glucose and urea, respectively. An appropriate amount of seaweed liquid fertilizer could significantly promote the growth of C. vulgaris. Three most important substrates for C. vulgaris were screened via Plackett-Burman experiments using Minitab software. Optimal concentrations of the three substrates were confirmed by Box-Behnken design and response surface analysis. The results were 58mg/L of dipotassium hydrogen phosphate, 162mg/L of magnesium sulfate and 198μL/L of seaweed liquid fertilizer. Cultivated with the optimized medium, dry cell weight of C. vulgaris after 48h reached 10.09g/L, increased by 61.2% compared with the original medium. The lipids yield was 3.62g/L, and the protein yield was 3.81g/L.
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