Abstract: The flocculation and separation methods were studied for oyster hydrolysate in this article, and the results showed that:86% impunity could be removed from the oyster hydrolysate by separator with 200L/h flow, 9360r/min and 15min flush off after pretreated under the special conditions, which to adjust the pH to 4.5 ～ 5.0, heated to 85℃ firstly and then cooling to 60℃.The amino acid was not too much changed, only the VBN had 25% decreased after separator, which also benefit to improve the flavor of oyster hydrolysate, and achieve to its quality requirement.Another, we also found that for the effluent factors:separator flow>pH>flocculent time>flocculent temperature.