Abstract: Clostridium kluyveri form caproic acid at anaerobic condition, major aroma caproic acid ethylester will be more outstanding if be used for fermentation of luzhou-flavor liquor distillate spirit.Diaphorase, phosphotransacetylase, thiolase, aldehyde dehydrogenase that come from metabolism of clostridium kluyveri at anaerobic condition can be used as biochemical reagent, detoxifying enzymes for toxicosis of acetone body, also be used for degradation of petroleum hydrocarbon pollutants, biological fuel cell and biosensor, and so on.The situation of study at home and abroad of acidogenic conditions of clostridium kluyveri and purification and purpose of these enzymes were summarized.