6种南瓜栽培品种体外抗氧化活性研究

doi:10.13386/j.issn1002-0306.2012.15.047

6种南瓜栽培品种体外抗氧化活性研究

Antioxidant activity of six varieties of Cucurbita moschata Duch. (pumpkin) cultivated in vitro

摘要

摘要: 目的:研究6种不同栽培品种南瓜（金钩、甜面、日本、超甜蜜本、蜜本、辽宁新民金钩）的体外抗氧化活性。方法:采用清除二苯代苦味酰基（DPPH）自由基、清除2,2’-联氨-（3-乙基苯并噻唑啉-6-磺酸）二氨盐（ABTS）自由基及铁离子还原/抗氧化能力（FRAP）测定法,评价南瓜体外抗氧化活性,并与阳性对照二丁基羟基甲苯（BHT）比较。结果与结论:南瓜体外抗氧化活性比较弱。六种样品中,超甜蜜本乙酸乙酯提取部分清除DPPH自由基（抑制率=38.99%）、ABTS自由基（IC50=20.90μg/mL）及还原Fe3+的能力（TEAC=（459.40±10.44）μmol/g）较好,抗氧化活性最强,但仍低于阳性对照BHT（清除率为93.93%,IC50=7.72μg/mL,TEAC=（1581.68±97.41）μmol/g）。

Abstract: Objective:To research antioxidant activity of 6 varieties of Cucurbita moschata Duch. (Jingou, Tianmian, Riben, Chaotianmiben, Miben and Liaoningxinminjingou) in vitro.Methods:1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, 2, 2'-azino-bis (3-ethylbenzothiazoline) -6-sulphonic aciddiamonium salt (ABTS) radical scavenging and ferric reducing antioxidant power (FRAP) assay were used to evaluated different varieties of C.moschata with BHT as positive control.Results and Conclusion:Antioxidant activity of C.moschata was not good.The DPPH radical scavenging (I%=38.99%) , ABTS radical scavenging (IC50=20.90μg/mL) , and ferric reducing antioxidant power (TEAC= (459.40± 10.44) μmol/g) of ethyl acetate extract of chaotianmiben C.moschata were higher than others.But it was still inferior to positive control BHT (I%=93.93%, IC50=7.72μg/mL, TEAC= (1581.68±97.41) μmol/g) .