徽州贡菊总黄酮对D半乳糖致衰老小鼠的抗氧化作用

Antioxidant effect of total flavonoids from Huizhou Gongju on aging mouse induced by D-galactose

摘要: 目的:探讨徽州贡菊总黄酮对衰老小鼠抗氧化作用的影响。方法:D-半乳糖致衰老模型法,采用200、400、800mg/（kg·dbw）剂量的徽州贡菊总黄酮给小鼠灌胃,检测血清和肝中超氧化物歧化酶（SOD）活性、谷胱甘肽过氧化物酶（GSH-Px）活性、过氧化氢酶（CAT）活性、总抗氧化能力（T-AOC）和血清、肝及脑中的MDA含量。结果:与模型组比较,不同剂量组能显著提高血清和肝组织中SOD、GSH-Px、CAT、T-AOC活性,降低血清、肝脏和脑组织中MDA含量。结论:徽州贡菊总黄酮具有一定的抗氧化功能。

Abstract: Objective:To research the anti-oxidative activity of total flavonoids from Gongju (GTF) in aged mice.Method:GTF was administrated by gavage200, 400, 800mg/ (kg·d bw) to mice for 30d.The mice were then sacrificed, and the serum, liver and brain tissue were collected.The concentration of malondialdehyde (MDA) in the blood, liver and brain tissue and the activities of superoxide dismutase (SOD) , glutathione peroxidase (GSH-Px) , Catalase (CAT) , total antioxidant activity (T-AOC) in the serum and liver were then measured.Result:GTF produced a dose-dependent decrease of MDA in the serum, liver and brain tissue, at the same time a dose-dependent increase of activities of SOD, GSH-Px, CAT and T-AOC at all three doses in the blood and liver.Conclusion:GTF had antioxidant activity by protecting body against free radical-induced damage.